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Independent samples t -tests were used for comparisons between two groups and one-way ANOVA followed by Bonferronis post hoc test was employed for comparisons among multiple groups

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Further investigation into the testicular microenvironment using a co-culture system of senescent TM3 Leydig cells and GC-1 spg spermatogonia revealed that H2O2-induced senescent TM3 cells secreted increased levels of SASP factors, particularly IL-6 and TGF-